Proponent/Claimant
Abstract
There is limited research on the attenuation of microorganisms, notably amoeba, in soil with biochar amendments. The purpose of this study is to determine the attenuation of amoeba in water-saturated clay and sandy soils containing biochar, as well as the effect of biochar on the transport of amoeba in clayey and sandy soils containing amoeba contamination. Four treatments were developed and duplicated three times: TS (sandy soil, control), TSB (sandy soil + biochar), TC (clayey soil, control), and TCB (clayey soil + biochar). The experiment was conducted using a 17cm 2.5cm diameter soil column; all treatments were saturated and leached. Under a compound microscope, the number of amoeba leached and attenuated was counted. Due to the narrower pores in clayey soil, it inhibited amoeba more effectively than sandy soil treatment. Additionally, biochar amendment in a sandy soil increases amoeba attenuation and decreases amoeba transport, whereas biochar supplementation in a clayey soil decreases amoeba attenuation and facilitates amoeba transfer. Additionally, it has been shown that the spatial distribution of amoeba is superior at a depth of four to eight centimeters in the soil column. According to the study, the physical augmentation of soil by biochar would be dependent on the starting texture of the soil. ' Ecology and Conservation, Biochar as a Filter for Amoeba Attenuation, Experiment on Amoeba Attenuation, Philippines